LOCUS       sequence-64507-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP5
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(117..241)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             294..338
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             348..371
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             372..395
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(450..471)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggggagagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
       61 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
      121 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
      181 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
      241 ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaaaagaaa
      301 ccgctgctgc taaattcgaa cgccagcaca tggacagcag cagcggcctg gaagttctgt
      361 tccaggggcc cctggaagtt ctgttccagg ggccctccgg agggatggtg agcaagggcg
      421 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      481 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      541 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      601 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      661 agtccgccat gcccgaa
//
