LOCUS       sequence-64508-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP5
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(65..71,72..89,90..95))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(120..244)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(322..341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(323..341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          367..570
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(390..410)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tttttctcca ttttagcttc cttagctcct gaaaatctcg acggatccta actcaaaatc
       61 cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc
      121 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
      181 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttt ttgtacaaac
      241 ttgttgatat ctgcagaatt ccaccacact ggactagtgg atccgagctc ggtaccaagc
      301 ttacctagcc agcttgggtc tccctatagt gagtcgtatt agtaccaagc taattcctca
      361 cgacacctga aatggaagaa aaaaactttg aaccactgtc tgaggcttga gaatgaacca
      421 agatccaaac tcaaaaaggg caaattccaa ggagaattac atcaagtgcc aagctggcct
      481 aacttcagtc tccacccact cagtgtgggg aaactccatc gcataaaacc cctcccccca
      541 acctaaagac gacgtactcc aaaagctcga gaactaatcg aggtgcctgg acggcgcccg
      601 gtactccgtg gagtcacatg aagcgacggc tgaggacgga aaggcccttt tcctttgtgt
      661 gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc tccctgcagc
      721 agggccggga agcggccatc tttccgctca cgcaactggt gccgaccggg ccagccttgc
      781 cgcccagggc ggggcgatac acggcggcgc gaggccaggc accagag
//
