LOCUS       sequence-64510-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann
            P, Brautigan DL, Stukenberg PT, Kapoor TM
  TITLE     Midzone activation of aurora B in anaphase produces an intracellular
            phosphorylation gradient.
  JOURNAL   Nature. 2008 Jun 19;453(7198):1132-6. doi: 10.1038/nature06923. Epub
            2008 May 7.
  PUBMED    18463638
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature06923"; journalName: "Nature"; date: "2008-06-19- 
            19"; volume: "453"; issue: "7198"; pages: "1132-6"
COMMENT     Sequence Label: CENP-B targeted Aurora B FRET sensor Kif2 subs...
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(281..283,284..796)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(323..344)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(584..603)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taaccgcatg atacagggta cgaaattcct tttacaagac ggagatgaaa tcaagatcat
       61 ttgggataaa aacaataaat ttgtcattgg ctttaaagtg gaaattaacg atactacagg
      121 tctgtttaac gagggattag gtatgttaca agaacaaaga gtagtactta agcaaacagc
      181 cgaagaaaaa gatttggtga aaaagttagg aggatccggc ggcaaggtga acaagatcgt
      241 gaagaaccgc cgcaccgtgg ccatcggtgg ttccggagga gtgagcaagg gcgaggagct
      301 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      361 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgct
      421 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
      481 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      541 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      601 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      661 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      721 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
      781 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
      841 catcggcgac g
//
