LOCUS       sequence-64518-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann
            P, Brautigan DL, Stukenberg PT, Kapoor TM
  TITLE     Midzone activation of aurora B in anaphase produces an intracellular
            phosphorylation gradient.
  JOURNAL   Nature. 2008 Jun 19;453(7198):1132-6. doi: 10.1038/nature06923. Epub
            2008 May 7.
  PUBMED    18463638
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature06923"; journalName: "Nature"; date: "2008-06-19- 
            19"; volume: "453"; issue: "7198"; pages: "1132-6"
COMMENT     Sequence Label: untargeted Aurora B FRET sensor Kif2 substrate
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(279..281,282..794)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(321..342)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(582..601)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 accgcatgat acagggtacg aaattccttt tacaagacgg agatgaaatc aagatcattt
       61 gggataaaaa caataaattt gtcattggct ttaaagtgga aattaacgat actacaggtc
      121 tgtttaacga gggattaggt atgttacaag aacaaagagt agtacttaag caaacagccg
      181 aagaaaaaga tttggtgaaa aagttaggag gatccggcgg caaggtgaac aagatcgtga
      241 agaaccgccg caccgtggcc atcggtggtt ccggaggagt gagcaagggc gaggagctgt
      301 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      361 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgctct
      421 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
      481 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      541 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      601 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      661 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      721 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      781 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
      841 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctgt
      901 tcaaagaccc caacgagaag cgcgatcaca tggtcctgct g
//
