LOCUS       sequence-64521-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann
            P, Brautigan DL, Stukenberg PT, Kapoor TM
  TITLE     Midzone activation of aurora B in anaphase produces an intracellular
            phosphorylation gradient.
  JOURNAL   Nature. 2008 Jun 19;453(7198):1132-6. doi: 10.1038/nature06923. Epub
            2008 May 7.
  PUBMED    18463638
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature06923"; journalName: "Nature"; date: "2008-06-19- 
            19"; volume: "453"; issue: "7198"; pages: "1132-6"
COMMENT     Sequence Label: Histone H2B targeted Aurora B FRET sensor Kif2...
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(183..893,894..896,897..899))
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(join(183..893,894..896,897..899))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(join(183..893,894..896,897..899))
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(225..246)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     574..593
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     regulatory      896..905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctttttga aaatgaagca atgaactcga gacaacctac tgtcttcaat tttacaattg
       61 caatcctcgg atctaccaat gaaaaatgga ttcacacctt gctgaatctc caggctttct
      121 tgaataatgc tgtcaggcaa tggttttaaa gttaaaaacc taccattacc cttggtacca
      181 gccttgtaca gctcgtccat gccgtgagtg atcccggcgg cggtcacgaa ctccagcagg
      241 accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt gctcaggtag
      301 tggttgtcgg gcaggatcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      361 gcgagctgca cgctgccgtc cgtgatgttg tggcgggcct tgaagtgggc cttgatgccg
      421 ttcttctgct tgtcggcggt gatatagacg ttgtggctga tgtagttgta ctccagcttg
      481 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      541 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      601 aagatggtac gctcctggac gtagccttcg ggcatgacgg acttgaagaa gtcgtgctgc
      661 ttcatgtggt cggggtagcg gctgaagcac tgcacgcccc aggtcagggt ggtcacgagg
      721 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      781 taggtggcat cgccctcgcc ctcgccggac acgctgaacc tgtggccgtt tacgtcgccc
      841 tccagctcga ccaggatggg cacgatcccg ccgaacagct cctcgccctt gctcaccatg
      901 gtggcgaa
//
