LOCUS       sequence-64530-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Srinivasan R, Pantoja R, Moss FJ, Mackey ED, Son CD, Miwa J, Lester 
            HA
  TITLE     Nicotine up-regulates alpha4beta2 nicotinic receptors and ER exit 
            sites via stoichiometry-dependent chaperoning.
  JOURNAL   J Gen Physiol. 2011 Jan;137(1):59-79.
  PUBMED    21187334
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol."; date: "2011-01"; volume: "137(1)"; pages: "59-79"
COMMENT     Sequence Label: pCI-neo:Beta2meGFP
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(480..731)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(522..543)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtctgctcga agcattaacc ctcactaaag ggaagcggcc gcccgggtcg actctagagg
       61 taccacgcgt gaattcgccc ttgaggagct gcaaatgaga gacctcactt ggagctggga
      121 gctgagtggt cagagtgaag gaaggtcgtg gcagtgtagt tctggaagag aggctgcagg
      181 aacatgccaa tggtcccaaa gacacagaca aagacaaaga tccagaggaa caggcggtcg
      241 atcaccatgg caacgtattt ccaatcctcc ctcacactct ggtcatcatc ttcacttcgc
      301 atatggtccg caatgaagcg tacaccgtcc acagcttccc ggaggccaca gctgcatggg
      361 cccatcgagc gccccagacc ggctgcagcg ggctcagcct ggaaagcccc agccaagccc
      421 tgcattgatg caggattgac aaagcaggta catgggtcag ctgcaggaca ggaccaggac
      481 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      541 atgtgatcgc gcttctcgtt ggggtctttg ctaagcttgg actgggtgct caggtagtgg
      601 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      661 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      721 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      781 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      841 gtgtcgccct cgaacttcac ctcggcgcgg g
//
