LOCUS       sequence-64545-e       10921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCLX-pTF-R1-DEST-R2-EBR65
FEATURES             Location/Qualifiers
     source          1..10921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2536..2554
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2577..2595
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2741..2759
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2844..2968
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2993..2998,2999..3016,3017..3023)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3077..3736
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3143..3162)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4078..4383
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4262..4281
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4424..4548)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     4585..4601
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4586..4602
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4617..4734
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4790..5001
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5020..5415
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5425..5484
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6547..6918
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6944..7532
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6997..7017)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7415..7426)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7674..7707)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7727..7907
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7945..7964)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8010..8065
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8064..8083)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8424..8440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8424..8440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8437..8459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8448..8464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8472..8478,8479..8496,8497..8502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8517..8538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8597..8792
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8643..8778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8798..8932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8835..8854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8889..8908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9000..9017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9171..9759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9251..9270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9930..10721,10722..10790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10553..10572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10791..10895)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttcg gtacaactcc
     2521 cactagacat atcctactct atcattgata gagtgaccct tccatagaca tatcctactc
     2581 tatcattgat agagtgaccc ttccactagg ttcacttttc tctatcattg atagggagtg
     2641 gtaaacaatt gatggacaaa atctatcaat gatagagagt gcatgttact agtattgttc
     2701 ttctctatca ttgatagtga ctttgcctaa tcacttagca actctatcat tgatagagtt
     2761 ctagtattag caaggccttt cctctatata aggaagttca tttcatttgg agaagatccc
     2821 cggggatcct ctagagtcga atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     2881 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     2941 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     3001 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     3061 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     3121 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     3181 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     3241 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     3301 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     3361 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     3421 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     3481 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     3541 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     3601 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     3661 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     3721 gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta
     3781 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     3841 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     3901 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     3961 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     4021 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     4081 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     4141 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     4201 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     4261 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     4321 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     4381 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     4441 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     4501 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtga ttcgacctgc
     4561 aggcatgcaa gctcgggccc cccctcgagg tcgacggtat cgataagctt gataactttt
     4621 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4681 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttccgatc
     4741 acgagactag cctcgagaag cttgatcgat ggctccggtg cccgtcagtg ggcagagcgc
     4801 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     4861 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4921 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4981 gggtttgccg ccagaacaca ggtgtcgtga cgcggatcca tggccaagcc tttgtctcaa
     5041 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     5101 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     5161 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     5221 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     5281 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     5341 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     5401 tatgtgtggg agggcggatc aggtcaatgt actaactacg ctttgttgaa gcttgccggc
     5461 gatgttgaaa gtaaccccgg tcctatgtcc agactggaca agagcaaagt cataaacggc
     5521 gctctggaat tactcaatgg agtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     5581 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     5641 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     5701 gagtcatggc aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca
     5761 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     5821 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     5881 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     5941 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     6001 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     6061 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccggc ctcgagcgaa
     6121 ttccagtacc tgccagatac agacgatcgt caccggattg aggagaaacg taaaaggaca
     6181 tatgagacct tcaagagcat catgaagaag agtcctttca gcggacccac cgacccccgg
     6241 cctccacctc gacgcattgc tgtgccttcc cgcagctcag cttctgtccc caagccagca
     6301 ccccagccct atccctttac gtcatccctg agcaccatca actatgatga gtttcccacc
     6361 atggtgtttc cttctgggca gatcagccag gcctcggcct tggccccggc ccctccccaa
     6421 gtcctgcccc aggctccagc ccctgcccct gctccagcca tggtatcagc tctggcccag
     6481 gccccagccc ctgtcccagt cctagcccca ggccctcctc aggctgtggc cccacctgcc
     6541 cccaagccca cccaggctgg ggaaggaacg ctgtcagagg ccctgctgca gctgcagttt
     6601 gatgatgaag acctgggggc cttgcttggc aacagcacag acccagctgt gttcacagac
     6661 ctggcatccg tcgacaactc cgagtttcag cagctgctga accagggcat acctgtggcc
     6721 ccccacacaa ctgagcccat gctgatggag taccctgagg ctataactcg cctagtgaca
     6781 ggggcccaga ggccccccga cccagctcct gctccactgg gggccccggg gctccccaat
     6841 ggcctccttt caggagatga agacttctcc tccattgcgg acatggactt ctcagccctg
     6901 ctgagtcaga tcagctccta aagatccgcg tcgtacggtc gacaatcaac ctctggatta
     6961 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7021 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7081 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7141 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7201 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7261 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7321 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     7381 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7441 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7501 gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga
     7561 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7621 gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg ggaataactt
     7681 cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt
     7741 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7801 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7861 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagttgaa
     7921 tcgccagtgt gtcgagctag cagatctttt tccctctgcc aaaaattatg gggacatcat
     7981 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     8041 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     8101 acatcagaat gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg
     8161 aacaaaggtg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     8221 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     8281 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     8341 tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc
     8401 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8461 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8521 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8581 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8641 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8701 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8761 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     8821 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8881 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     8941 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9361 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9601 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10921 g
//
