LOCUS       sequence-64546-e       11023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 11023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCWX-R4-DEST-R2-PGR65
FEATURES             Location/Qualifiers
     source          1..11023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2645
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2670..2675,2676..2693,2694..2700)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2754..3413
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2820..2839)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3755..4060
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3939..3958
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4115..4239)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4257..4767
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4686..4704
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4783..4792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4789..4791,4792..4794,4795..5505)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4789..4791,4792..4794,4795..5505)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4834..4855)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5095..5114)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5442..5463
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5521..5580
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6649..7020
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    7040..7628
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7093..7113)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7511..7522)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7631..7647)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7632..7648)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7776..7809)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7829..8009
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8047..8066)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8112..8167
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8166..8185)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8526..8542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8526..8542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8539..8561)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8550..8566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8574..8580,8581..8598,8599..8604))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8619..8640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8699..8894
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8745..8880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8900..9034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8937..8956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8991..9010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9102..9119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9273..9861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9353..9372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10032..10823,10824..10892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10655..10674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10893..10997)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta
     2581 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     2641 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     2701 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
     2761 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     2821 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     2881 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     2941 ttgcccgcct gatgaatgct catccggaac tccgtatggc aatgaaagac ggtgagctgg
     3001 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     3061 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     3121 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     3181 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     3241 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     3301 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     3361 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
     3421 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     3481 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     3541 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     3601 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3661 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3721 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     3781 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     3841 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     3901 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     3961 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     4021 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     4081 cagccagtct gcaggtcgag cagatctgct cgaccatagt gactggatat gttgtgtttt
     4141 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     4201 attttacgtt tctcgttcag ctttcttgta caaagtggtg ataatcgaat tcccacgggg
     4261 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     4321 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     4381 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     4441 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     4501 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     4561 cgaccgcgat gggctgtggc caatagcggc tgctcagcgg ggcgcgccga gagcagcggc
     4621 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     4681 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     4741 gaccgaatca ccgacctctc tccccagggg gatccaccgg tcgccaccat ggtgagcaag
     4801 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4861 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     4921 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4981 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5041 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5101 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5161 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5221 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5281 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     5341 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     5401 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5461 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggctag cggatcaggt
     5521 caatgtacta actacgcttt gttgaagctt gccggcgatg ttgaaagtaa ccccggtcct
     5581 ggatccatgt ccagactgga caagagcaaa gtcataaacg gcgctctgga attactcaat
     5641 ggagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     5701 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag
     5761 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     5821 ctgcggaaca acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa
     5881 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     5941 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     6001 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga
     6061 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     6121 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     6181 aaacagctaa agtgcgaaag cggcgggccg gcctcgagcg aattccagta cctgccagat
     6241 acagacgatc gtcaccggat tgaggagaaa cgtaaaagga catatgagac cttcaagagc
     6301 atcatgaaga agagtccttt cagcggaccc accgaccccc ggcctccacc tcgacgcatt
     6361 gctgtgcctt cccgcagctc agcttctgtc cccaagccag caccccagcc ctatcccttt
     6421 acgtcatccc tgagcaccat caactatgat gagtttccca ccatggtgtt tccttctggg
     6481 cagatcagcc aggcctcggc cttggccccg gcccctcccc aagtcctgcc ccaggctcca
     6541 gcccctgccc ctgctccagc catggtatca gctctggccc aggccccagc ccctgtccca
     6601 gtcctagccc caggccctcc tcaggctgtg gccccacctg cccccaagcc cacccaggct
     6661 ggggaaggaa cgctgtcaga ggccctgctg cagctgcagt ttgatgatga agacctgggg
     6721 gccttgcttg gcaacagcac agacccagct gtgttcacag acctggcatc cgtcgacaac
     6781 tccgagtttc agcagctgct gaaccagggc atacctgtgg ccccccacac aactgagccc
     6841 atgctgatgg agtaccctga ggctataact cgcctagtga caggggccca gaggcccccc
     6901 gacccagctc ctgctccact gggggccccg gggctcccca atggcctcct ttcaggagat
     6961 gaagacttct cctccattgc ggacatggac ttctcagccc tgctgagtca gatcagctcc
     7021 taagatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7081 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7141 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7201 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7261 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7321 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7381 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7441 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7501 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7561 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7621 ctccccgcat cgataccgtc gacctcgagg ttcgctttaa gaccaatgac ttacaaggca
     7681 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7741 caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc atacattata
     7801 cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7861 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7921 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7981 cttttagtca gtgtggaaaa tctctagcag catctagttg aatcgccagt gtgtcgagct
     8041 agcagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     8101 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     8161 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     8221 ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa
     8281 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     8341 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     8401 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     8461 tcatagctgt ccctcttctc ttatgaagat ccctcgacct gcagcccaag cttggcgtaa
     8521 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8581 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8641 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     8701 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8821 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8881 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8941 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9001 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     9061 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     9481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     9721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9901 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10021 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10081 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10141 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10201 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10261 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10321 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10381 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10441 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10501 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10561 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10621 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10681 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10741 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10801 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10861 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10921 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10981 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
