LOCUS       sequence-64552-e        3878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Giry-Laterriere M, Verhoeyen E, Salmon P
  TITLE     Lentiviral vectors.
  JOURNAL   Methods Mol Biol. 2011;737:183-209. doi: 
            10.1007/978-1-61779-095-9_8.
  PUBMED    21590398
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2011"; volume: "737:183-209. doi"; pages: " 
            10.1007/978-1-61779-095-9_8"
COMMENT     Sequence Label: pENTR-L4-UBI-L1R
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     96..118
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     110..127
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    167..262
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        280..1490
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1327..1346
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1498..1621
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1693..1712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1694..1712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1717..1733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1717..1733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1835..1854)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1846..2655
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1913..1932)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2802..3390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3291..3310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3544..3561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3720..3747)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(join(3839..3878,1..47))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt
       61 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
      121 ggccagtctt aagctcgggc ccgcgttaac gctaccatgg agctccaaat aatgatttta
      181 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt ttttataatg
      241 ccaactttgt atagaaaagt tgttaattaa gggtgcagcg gcctccgcgc cgggttttgg
      301 cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca
      361 ggagcgttcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact
      421 cggccttaga accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta
      481 gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg
      541 attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg
      601 gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc
      661 gctgtgatcg tcacttggtg agttgcgggc tgctgggctg gccggggctt tcgtggccgc
      721 cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc
      781 gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc acaaaatggc ggctgttccc
      841 gagtcttgaa tggaagacgc ttgtaaggcg ggctgtgagg tcgttgaaac aaggtggggg
      901 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
      961 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     1021 ggagaactcg ggtttgtcgt ctggttgcgg gggcggcagt tatgcggtgc cgttgggcag
     1081 tgcacccgta cctttgggag cgcgcgcctc gtcgtgtcgt gacgtcaccc gttctgttgg
     1141 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     1201 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     1261 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     1321 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     1381 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     1441 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac aggatcccaa
     1501 ctttgtacaa aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     1561 agattttgca taaaaaacag actacataat actgtaaaac acaacatatg cagtcactat
     1621 gaatcaacta cttagatggt attagtgacc tgtagaattc gagctctaga gctgcagggc
     1681 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1741 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1801 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1861 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1921 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1981 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2041 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2101 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2161 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2221 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2281 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2341 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2401 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2461 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2521 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2581 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2641 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2701 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2761 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2821 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2881 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2941 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3001 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3061 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3121 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3181 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3241 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3301 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3361 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3421 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3481 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3541 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3601 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3661 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3721 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3781 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3841 ttgtcctact caggagagcg ttcaccgaca aacaacag
//
