LOCUS       sequence-64563-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, 
            Lampson MA
  TITLE     Regulated targeting of protein phosphatase 1 to the outer 
            kinetochore by KNL1 opposes Aurora B kinase.
  JOURNAL   J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. 
            Epub 2010 Mar 15.
  PUBMED    20231380
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201001006"; journalName: "J Cell Biol"; date: 
            "2010-03-22- 22"; volume: "188"; issue: "6"; pages: "809-20"
COMMENT     Sequence Label: CENP-B mCherry PP1 gamma
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      642..651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(672..691)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(673..691)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(736..835)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(765..785)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
       61 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
      121 caccatggtg gcgaagcatg ctagcccgcc actgccctcc gagggcaccg cggccggact
      181 ggcaggcgcc gccggggtgc ggggggcagc gtttcgcgcg cgggcgcggg ccacgccgct
      241 gcaggacacc acgccgtggc gccggcggaa gcggtccagc cagccgttgg aggcggtgaa
      301 gtcgtccatg cccagctcct cggctatgcg cagcgccttc tccttgagga tgatgccctt
      361 gaccggcagg ccggcggcgc ggatctgctg gaaccaggcg atgagcaagc cctcgagctt
      421 gtcgtaggga gacagcttgt tggtcttgcg gcaggtggag gccaccccgt acttgcgctc
      481 cgacgccagg atggcgcgct tgttcttcag gatcgtgctc agcgtggacg gcgggatgtt
      541 gaagcgccgc gcgatctcgc ccttgcgcag gtccggattc tcctccacct cctggatgat
      601 ccgtgacttc tcccggaacg tcagctgtcg cctcttgggg cccatggtgg cggcgcgcca
      661 agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc
      721 tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      781 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      841 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
      901 ct
//
