LOCUS       sequence-64572-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, 
            Lampson MA
  TITLE     Regulated targeting of protein phosphatase 1 to the outer 
            kinetochore by KNL1 opposes Aurora B kinase.
  JOURNAL   J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. 
            Epub 2010 Mar 15.
  PUBMED    20231380
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201001006"; journalName: "J Cell Biol"; date: 
            "2010-03-22- 22"; volume: "188"; issue: "6"; pages: "809-20"
COMMENT     Sequence Label: C-term Hec1-targeted Aurora B FRET sensor
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggc gcgccatgaa gcgcagttca gtttccagcg
      121 gtggtgctgg ccgcctctcc atgcaggagt taagatccca ggatgtaaat aaacaaggcc
      181 tctacacccc tcaaaccaaa gagaaaccaa cctttggaaa gttgagtata aacaaaccga
      241 catctgaaag aaaagtctcg ctatttggca aaagaactag tggacatgga tcccggaata
      301 gtcaacttgg tatattttcc agttctgaga aaatcaagga cccgagacca cttaatgaca
      361 aagcattcat tcagcagtgt attcgacaac tctgtgagtt tcttacagaa aatggttatg
      421 cacataatgt gtccatgaaa tctctacaag ctccctctgt taaagacttc ctgaagatct
      481 tcacatttct ttatggcttc ctgtgcccct catacgaact tcctgacaca aagtttgaag
      541 aagaggttcc aagaatcttt aaagaccttg ggtatccttt tgcactatcc aaaagctcca
      601 tgtacacagt gggggctcct catacatggc ctcacattgt ggcagcctta gtttggctaa
      661 tagactgcat caagatacat actgccatga aagaaagctc acctttattt gatgatgggc
      721 agccttgggg agaagaaact gaagatggaa ttatgcataa taagttgttt ttggactaca
      781 ccataaaatg ctatgagagt tttatgagtg gtgccgacag ctttgatgag atgaa
//
