LOCUS       sequence-64577-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM
  TITLE     Sensing chromosome bi-orientation by spatial separation of aurora B 
            kinase from kinetochore substrates.
  JOURNAL   Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. 
            Epub 2009 Jan 15.
  PUBMED    19150808
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1167000"; journalName: "Science"; date: "2009-03-6-
            6"; volume: "323"; issue: "5919"; pages: "1350-3"
COMMENT     Sequence Label: nonphosphorylatable Mis12-targeted Aurora B FR...
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      729..738
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggc gcgccaccat gtctgtggat ccaatgacct
      121 acgaggccca gttctttggc ttcacgccac aaacgtgcat gcttcggatc tacattgcat
      181 ttcaagacta cctatttgaa gtgatgcagg ccgttgaaca ggttattctg aagaagctgg
      241 atggcatccc agactgtgac attagcccag tgcagattcg caaatgcaca gagaagtttc
      301 tttgcttcat gaaaggacat tttgataacc tttttagcaa aatggagcaa ctgtttttgc
      361 agctgatttt acgtattccc tcaaacatct tgcttcctga agataaatgt aaggagacac
      421 cttatagtga ggaagatttt cagcatctcc agaaagaaat tgaacagtta caggagaagt
      481 acaagactga attatgtact aagcaggccc ttcttgcaga attagaagag caaaaaattg
      541 ttcaggccaa actcaaacag acgttgactt tctttgatga gcttcataat gttggcagag
      601 atcatgggac tagtgatttt agggagagtt tagtatccct ggttcagaac tccagaaaac
      661 tacagaacat tagagacaat gtggaaaagg aatcgaaacg actgaaaata tctatgctag
      721 cctcgagcgc caccatggtg agcaagggcg aggagaccac aatgggcgta atcaagcccg
      781 acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc gtgatcgagg
      841 gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag gtgaaggagg
      901 gagcccccct gccct
//
