LOCUS       sequence-64578-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ908
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(147..452)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(249..268)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
       61 aacagactac ataatactgt aaaacacaac atatccagtc actatggtcg acctgcagac
      121 tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt aatggcgttt
      181 ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc
      241 acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg
      301 taaagttcac gggggacttt atctgacagc agacgtgcac tggccagggg gatcaccatc
      361 cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc
      421 tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcggcaaa
      481 agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt
      541 ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta ccgaaccgga
      601 gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta
      661 atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata tcagtatata
      721 ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tttagtaagc
      781 cggatcctct agattacgcc ccgcctgcca ctcatcgcag tactgttgta attcattaag
      841 cattctg
//
