LOCUS       sequence-64579-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1000
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..448)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(245..264)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
       61 gactacataa tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc
      121 tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga
      181 tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac
      241 tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa
      301 gttcacgggg gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc
      361 gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc
      421 ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc ggcaaaagag
      481 ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag
      541 cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccga accggagata
      601 ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac
      661 gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag tatatattct
      721 tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga
      781 tcctctagat tacgccccgc ctgccactca tcgcagtact gttgtaattc attaagcatt
      841 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatca
//
