LOCUS       sequence-64582-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1002
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..453)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(250..269)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
       61 aaacagacta cataatactg taaaacacaa catatccagt cactatggtc gacctgcaga
      121 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      181 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
      241 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      301 gtaaagttca cgggggactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
      361 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
      421 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcggcaa
      481 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
      541 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accgaaccgg
      601 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
      661 aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat
      721 attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
      781 ccggatcctc tagattacgc
//
