LOCUS       sequence-64585-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1202
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    7..114
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     31..47
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     31..47
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(81..97)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(82..98)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(114..134)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(142..159)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(142..158)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaagttgagc tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag
       61 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccaacttt
      121 attatacata gttgataatt cactggccgt cgttttacag gccggatggg ccatggcacc
      181 ttaacatttc taaattttag tttaataaaa aaattttaaa ataatacgag ttttgagact
      241 aactttcaat aaaaaccaaa aaattgcttc aaaaaagagc tcgcgccgat ttacgataaa
      301 cacagcgcgc tacgacactc cgaaaaccgc gccgcaccat tacattctcc gccccaccgc
      361 acggtttgcg gaaggcgatg aaattgctgg gcctaaaatt ttaacccttt ttttcccgtt
      421 tttcaactgt tttcccgatt ttttcgatat ttttttcagg tttttagtta gaaaagtata
      481 taaaaattgc attatttgta gttttcgctc gtaatttgtg atagaaaacg gccaatttgc
      541 ctagatttgt gattctacca agtggaattt ttgggaaaat atcaatattc cacgattttt
      601 attcaaatc
//
