LOCUS       sequence-64588-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1200
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    3..110
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     27..43
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..43
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(77..93)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(78..94)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(110..130)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(138..155)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(138..154)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(769..788)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ttgagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat
       61 tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc aactttatta
      121 tacatagttg ataattcact ggccgtcgtt ttacagaggg gagaaaatgc ggagagaaga
      181 acccagcatc ttaataaaat tatataattt tgaataaaat caaattaaaa ataagattaa
      241 ttgaaacaca acaatagaat gcagaaaatt actcataaga aataatttat tttgaaagaa
      301 gttgtgtcga tttacgataa aaactacacg caaacgaggc tccgcgttta caccgtttgg
      361 caatggagcg cgcttgctat tagattttag gccagctcaa cgaaagttta atttattttt
      421 cttctttttt tccatttttt tgagtaaaat atcgaatttt ttgtattaat tctgtaaatt
      481 ttataatttg caactatttt ggcgtattct gcttgttttt atcagcgaat ttttatgcat
      541 ttcagtcaaa agtttgagca ggaaaaatga catttaggac tttgtaaaat tgtattctta
      601 aatttgcttg aaaacctacg tgtacaattg attttattga atttttctta cgtttcccgt
      661 tgtatcttat tgtatttaac tttcatcata aaataattta tttccaggta aaatatccga
      721 cgctctcgtg gatccccggg attggctcag aaaaaaaagg taccgaccat gaccgagtac
      781 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
      841 gcc
//
