LOCUS       sequence-64593-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1324
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           75..117
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     protein_bind    120..144
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(293..304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctttgtatag aaaagttggt gaattgtaat acctcactat agggcgaatt aattcgagct
       61 cggtacccag cttgcttgtt ctttttgcag aagctcagaa taaacgctca actttggcag
      121 caagtttgta caaaaaagca ggctctttga gccaatttat ccaagtcctt gtaaaaagta
      181 ttcgaaaatt gttaacggat aaaatgttta ttataatatc aaaaacaatt tgtcagttga
      241 ccactttttg atatatttga cagaaaccgg gatgaattgg ctcaaagtta gggcgccctt
      301 ctattacagg gtttctgata acaaaccggt tattaactcc caacaaggga tgatttcaat
      361 tcatcatgct caaattgacc caaattaagt tacatgacaa attcatcgcc cttttcaact
      421 cttcttggct catcatctgt tattctgttc ctattctgtc tgcaccctat acccctttgc
      481 atactctctc gtcattcctt tcttggatag tgtcttcttc tccccagctc tgctacttct
      541 atgacttgcg cccgctgctt ttccgctcgt tcctctcctc tctgacgtct tcttctgctt
      601 cttttctcct tcctttttcc cttctcaatc ttcctctttt tcccatttct gtcaatcatt
      661 cgaagaagaa gaagaagaag accctcattc atttcatttt tttttctgtc ggtgtgtgct
      721 gctcggttaa gagtgagctc tctattccac gtcttcttct ttttcttctt cgattcgaat
      781 caatcactcc accaaaacgc attcgttttt gggattcacc ccgcggttcg caa
//
