LOCUS       sequence-64606-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, 
            Lampson MA
  TITLE     Regulated targeting of protein phosphatase 1 to the outer 
            kinetochore by KNL1 opposes Aurora B kinase.
  JOURNAL   J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. 
            Epub 2010 Mar 15.
  PUBMED    20231380
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201001006"; journalName: "J Cell Biol"; date: 
            "2010-03-22- 22"; volume: "188"; issue: "6"; pages: "809-20"
COMMENT     Sequence Label: GFP KNL1 wt hardened
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             104..148
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtccgg aggggatcgg tggagctcca ccggtggcgg
       61 ccgctctaga gagaatcttt attttcaggg cgccgccaaa ttcaaagaaa ccgctgctgc
      121 taaattcgaa cgccagcaca tggacagcgg aggtggaggt actagtctcg agatggatgg
      181 ggtgtcttca gaggctaatg aagaaaatga caatatagag agacctgtta gaagacggca
      241 ttcttcaata ttgaaacccc caaggagtcc tcttcaggac ctcagaggtg ggaatgaaac
      301 agttcaagag tcaaacgcgt taaggaataa gaaaaactct cgtcgagtca gctttgcaga
      361 tactataaag gtattccaga cggagtctca tatgaaaata gtgagaaagt cagaaatgga
      421 agaaacagaa gcaggagaaa atcttctttt gatacagaat aagaaattag aagataatta
      481 ctgtgaaatt actgggatga acacattgct ttctgctccc attcataccc agatgcaaca
      541 gaaggagttt tcaattatag aacatacccg tgaaaggaaa catgcaaatg accagacagt
      601 cattttttca gatgaaaacc agatggacct gacatcaagt cacactgtaa tgattaccaa
      661 aggcctttta gataatccca taagtggaaa gtccaccaag atagatacca catcatttct
      721 agctaattta aagcttcaca ccgaggactc aagaatgaaa aaagaagtaa atttttccgt
      781 ggatcaaaac acttcttcag aaaataaaat agatttcaat gacttcataa aaagattgaa
      841 aacaggaaaa tgtagt
//
