LOCUS       sequence-64608-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, 
            Lampson MA
  TITLE     Regulated targeting of protein phosphatase 1 to the outer 
            kinetochore by KNL1 opposes Aurora B kinase.
  JOURNAL   J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. 
            Epub 2010 Mar 15.
  PUBMED    20231380
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201001006"; journalName: "J Cell Biol"; date: 
            "2010-03-22- 22"; volume: "188"; issue: "6"; pages: "809-20"
COMMENT     Sequence Label: GFP KNL1 wt hardened and RVSF->AAAA
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             105..149
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tctcgggcat ggacgagctg tacaagtccg gaggggatcg gtggagctcc accggtggcg
       61 gccgctctag agagaatctt tattttcagg gcgccgccaa attcaaagaa accgctgctg
      121 ctaaattcga acgccagcac atggacagcg gaggtggagg tactagtctc gagatggatg
      181 gggtgtcttc agaggctaat gaagaaaatg acaatataga gagacctgtt agaagacggc
      241 attcttcaat attgaaaccc ccaaggagtc ctcttcagga cctcagaggt gggaatgaaa
      301 cagttcaaga gtcaaacgcg ttaaggaata agaaaaactc tcgtgcagcc gccgctgcag
      361 atactataaa ggtattccag acggagtctc atatgaaaat agtgagaaag tcagaaatgg
      421 aagaaacaga agcaggagaa aatcttcttt tgatacagaa taagaaatta gaagataatt
      481 actgtgaaat tactgggatg aacacattgc tttctgctcc cattcatacc cagatgcaac
      541 agaaggagtt ttcaattata gaacataccc gtgaaaggaa acatgcaaat gaccagacag
      601 tcattttttc agatgaaaac cagatggacc tgacatcaag tcacactgta atgattacca
      661 aaggcctttt agataatccc ataagtggaa agtccaccaa gatagatacc acatcatttc
      721 tagctaattt aaagcttcac accgaggact caagaatgaa aaaagaagta aatttttccg
      781 tggatcaaaa cacttcttca gaaaataaaa tagatttcaa tgact
//
