LOCUS       sequence-64617-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Chadwick BP, Willard HF
  TITLE     A novel chromatin protein, distantly related to histone H2A, is 
            largely excluded from the inactive X chromosome.
  JOURNAL   J Cell Biol. 2001 Jan 22;152(2):375-84.
  PUBMED    11266453
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2001-01-22"; volume: "152(2)"; pages: "375-84"
COMMENT     Sequence Label: H2A-Bbd-MYC
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(34..63)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(531..550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(532..550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(595..798)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(624..644)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggtgatgg tgatgatgac cggtatgcat attcagatcc tcttctgaga tgagtttttg
       61 ttcgaagggc cctctagact cgagcggccg ccactgtgct ggatatctgc agaattcgtc
      121 ctcgccaggg gccacttgag agatggtggt cgtgttgaaa agggtgctca gtagcctgtc
      181 gttgtgaacc accatgtcca gcagcagggg agtgatgttc cgctctccgc tgttctgggc
      241 ctcgttgccc gccagctcca ggaccttggc cgtcaggtac tcaataaccg cagcgaggta
      301 gaccggcgcc gtgcgactca ggcgctgagc gtagtggccc tcccgtagac tgcgctccac
      361 ctggctcact gaaaacgaaa gctccgctcg gacggtgcga gagcaggtcc gcccccggcc
      421 gccagcaccg gaggaccctc ggcgtctcct cctcctcggc atcagaattc caccacactg
      481 gactagtgga tccgagctcg gtaccaagct taactagcca gcttgggtct ccctatagtg
      541 agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct
      601 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
      661 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
      721 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
      781 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
      841 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
      901 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
      961 ttaccgtaaa ta
//
