LOCUS       sequence-64618-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Chadwick BP, Willard HF
  TITLE     A novel chromatin protein, distantly related to histone H2A, is 
            largely excluded from the inactive X chromosome.
  JOURNAL   J Cell Biol. 2001 Jan 22;152(2):375-84.
  PUBMED    11266453
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2001-01-22"; volume: "152(2)"; pages: "375-84"
COMMENT     Sequence Label: H2A-Bbd-GFP
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..711,712..714,715..717))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(41..61)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     656..684
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttgtagagc tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac
       61 catgtggtca cgcttttcgt tgggatcttt cgaaagggca gattgtgtcg acaggtaatg
      121 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      181 tagttgaacg gatccatctt caatgttgtg gcgaattttg aagttagctt tgattccatt
      241 cttttgtttg tctgccgtga tgtatacatt gtgtgagtta tagttgtact cgagtttgtg
      301 tccgagaatg tttccatctt ctttaaaatc aatacctttt aactcgatac gattaacaag
      361 ggtatcacct tcaaacttga cttcagcacg cgtcttgtag ttcccgtcat ctttgaaaga
      421 tatagtgcgt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt
      481 catatgatcc ggataacggg aaaagcattg aacaccataa gagaaagtag tgacaagtgt
      541 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gctttccgta
      601 tgtagcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      661 taattcaaca agaattggga caactccagt gaaaagttct tctcctttgc tagccattct
      721 agactcgagc ggccgccact gtgctggata tctgcagaat tcgtcctcgc caggggccac
      781 ttgagagatg gtggtcgtgt tgaaaagggt gctcagtagc ctgtcgttgt gaaccaccat
      841 gtccagcagc aggggagtga tgttccgctc tccgctgttc tggg
//
