LOCUS       sequence-64637-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  TITLE     Sean Wu lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNK-TGCK
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     224..245
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      287..296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(414..438)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    465..483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    507..525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    549..567
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    591..609
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    633..651
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
       61 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
      121 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
      181 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      241 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc
      301 ggtgcagatc tagactcgag aagctttaag tgcaggaatt cggggacgcg gaggctggat
      361 cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga
      421 cggttcacta aacgagctct gcttatatag gtcgagttta ccactcccta tcagtgatag
      481 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      541 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
      601 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      661 gtcgagctcg gtaccgaatt caacaggcat ctactgagtg gacccaacgc atgagaggac
      721 agtgccaagc aagcaactca aatgtcccac cggttgggca tggccaggta gcctatgctg
      781 tgtctggacg tcctcctgct ggtatagtta ttttaaaatc agaaggacag ggaagggagc
      841 agtggttcac gcctgtaatc ccagcaattt gggaggccaa ggtgggtaga tcacctgaga
      901 ttaggagttg gagaccagcc tggccaatat ggtgaaaccc cgtctctacc aaaaaaacaa
      961 aaattagctg agcctggtca tgcatgcctg gaatcccaac aactcgggag gctgaggcag
     1021 gagaatcgct tga
//
