LOCUS       sequence-64660-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu
            K, Bokoch GM, Hahn KM
  TITLE     Rho family proteins modulate rapid apoptosis induced by cytotoxic T 
            lymphocytes and Fas.
  JOURNAL   J Biol Chem. 2000 Mar 31. 275(13):9725-33.
  PUBMED    10734125
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 31. 275(13):9725-33."
COMMENT     Sequence Label: pcDNA3-EGFP-Cdc42-T17N
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(34..558,559..561,562..609))
                     /codon_start=1
                     /product="dominant negative mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (T17N)
                     /translation="MQTIKCVVVGDGAVGKNCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     primer_bind     complement(658..679)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagtcatagc agcacacacc tgcggctctt
       61 cttcggttct ggaggctcca gggcagccaa tattgcttcg tcaaatacat tctttaggcc
      121 tttctgtgta agtgcagaac actccacata cttgacagcc ttcaggtcac gggccagctt
      181 ttcagcagtc tctggagtga taggcttctg tttgttcttg gcaagtttct caatagtaga
      241 ggggtcatct ctgagatcaa tttgagtccc aacaagcaag aaaggagtct ttggacagtg
      301 gtgagttatc tcaggcaccc acttttcttt cacgttttca aatgaagatg gagagaccac
      361 tgaaaaacag actagaaata catctgtttg tggataactc agcggtcgta atctgtcata
      421 atcctcttgc cctgcagtat caaaaagtcc aagagtatat ggctctccac caatcataac
      481 tgtgactgca tagttgtcaa aaacagtcgg tacatattcc gatggaaatt tgtttgttgt
      541 gtaggatatc aggagacaat ttttaccaac agcaccatcg cccacaacaa cacacttaat
      601 tgtctgcatg aattccttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      661 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      721 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      781 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      841 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      901 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtc
//
