LOCUS       sequence-64662-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Yang YS, Hughes TE
  TITLE     Cre stoplight: a red/green fluorescent reporter of Cre recombinase 
            expression in living cells.
  JOURNAL   Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.
  PUBMED    11730010
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2001-11"; volume: "31(5)"; pages: "1036, 
            1038, 1040-1"
COMMENT     Sequence Label: Cre Stoplight
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(83..757,758..760,761..763))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(145..168)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(343..362)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     560..580
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      760..769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    799..832
                     /label=loxH
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021). The loxH variant 
                     was optimized for expression constructs but remains 
                     compatible with loxP."
     primer_bind     complement(851..870)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(852..870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(915..966)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(944..964)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtgatgatg gctagaatcg agaccgagga gagggttagg gataggctta ccgagctccc
       61 gaattgccct tagtcgaggc cgctacagga acaggtggtg gcggccctcg gtgcgctcgt
      121 actgctccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg gagtccacgt
      181 agtagtagcc gggcagctgc acgggcttct tggccatgta gatggacttg aactccacca
      241 ggtagtggcc gccgtccttc agcttcaggg ccttgtggat ctcgcccttc agcacgccgt
      301 cgcgggggta caggcgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg
      361 ggccgtcgga ggggaagttc acgccgatga acttcacctt gtagatgaag cagccgtcct
      421 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
      481 cccacttgaa gccctcgggg aaggacagct tcttgtagtc ggggatgtcg gcggggtgct
      541 tcacgtacac cttggagccg tactggaact ggggggacag gatgtcccag gcgaagggca
      601 gggggccgcc cttggtcacc ttcagcttca cggtgttgtg gccctcgtag gggcggccct
      661 cgccctcgcc ctcgatctcg aactcgtggc cgttcacggt gccctccatg cgcaccttga
      721 agcgcatgaa ctccttgatg acgttcttgg aggagcgcac catggtggca agggcaattc
      781 ctcgagccaa ttcctccgat aacttcgtat aatgtatgct atatgaggta attctagcca
      841 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
      901 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      961 tgcgtcaatg gggcgga
//
