LOCUS       sequence-64663-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Lei K, Nimnual A, Zong WX, Kennedy NJ, Flavell RA, Thompson CB, 
            Bar-Sagi D, Davis RJ
  TITLE     The Bax subfamily of Bcl2-related proteins is essential for 
            apoptotic signal transduction by c-Jun NH(2)-terminal kinase.
  JOURNAL   Mol Cell Biol. 2002 Jul . 22(13):4929-42.
  PUBMED    12052897
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2002 Jul . 22(13):4929-42."
COMMENT     Sequence Label: pCDNA3 Flag MKK7B2Jnk2a2
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(245..256)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt cggcagccaa catggactat aaggacgatg atgacaaaat
      121 ggcggcgtcc tccctggagc agaagctgtc ccgcctggaa gccaagctga agcaggagaa
      181 ccgtgaggcc cgcaggagga tcgacctcaa cttggatatc agcccacagc ggcccaggcc
      241 caccctgcaa ctcccactgg ccaacgatgg gggcagccgc tcaccatcct cagagagctc
      301 cccacagcac cctacacccc ccacccggcc ccgccacatg ctggggctcc catcaacctt
      361 gttcacaccg cgcagtatgg agagcatcga gattgaccag aagctgcagg agatcatgaa
      421 gcagacaggg tacctgacta tcgggggcca gcgttatcag gcagaaatca atgacttgga
      481 gaacttgggt gagatgggca gtggtacctg tggtcaggtg tggaagatgc ggttccggaa
      541 gacaggccac atcattgctg ttaagcaaat gcggcgctct gggaacaagg aagagaataa
      601 gcgcattttg atggacctgg atgtagtact caagagccat gactgccctt acatcgttca
      661 gtgctttggc accttcatca ccaacacaga cgtctttatt gccatggagc tcatgggcac
      721 atgtgcagag aagctgaaga aacgaatgca gggccccatt ccagagcgaa tcctgggcaa
      781 gatgactgtg gcgattgtga aagcactgta ctatctga
//
