LOCUS       sequence-64669-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     Gourisankar Ghosh unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET11a-p65(19-325)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgcctta tgtggagatc atcgaacagc cgaagcaacg
      181 gggcatgcga ttccgctata aatgcgaggg gcgctcagcg ggcagtattc ctggcgagag
      241 aagcacagat accaccaaga cacaccccac catcaagatc aatggctaca caggaccagg
      301 aacagttcga atctccctgg tcaccaagga tccacctcac cggcctcatc cacatgaact
      361 tgtggggaag gactgccggg atggctacta tgaggctgac ctctgcccag accgcagtat
      421 ccatagcttc cagaacctgg ggatccagtg tgtgaagaag cgagacctgg agcaagccat
      481 tagccagcga atccagacca acaataaccc ctttcacgtt cctatagagg agcagcgcgg
      541 ggactatgac ttgaatgcag tgcgcctctg cttccaggtg acagtgcggg acccagcagg
      601 caggcccctc ctcctgaccc ctgtcctctc acatccgatt tttgataacc gggcccccaa
      661 cactgccgag ctcaagatct gccgagtaaa ccggaactct gggagctgcc tcggtgggga
      721 tgagatcttc ttgctgtgcg acaaggtgca gaaagaagac attgaggtgt atttcacggg
      781 accaggctgg gaggcacgag gctccttttc tcaagctgat gtgcatcggc aagtggccat
      841 tgtgttccgg actcctccgt acgccgaccc
//
