LOCUS       sequence-64677-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Gourisankar Ghosh unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-His-IkappaBalpha(FL)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             178..195
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg
      181 gtgccgcgcg gcagccatat gttccaggcg gccgagcgcc cccaggagtg ggccatggag
      241 ggcccccgcg acgggctgaa gaaggagcgg ctactggacg accgccacga cagcggcctg
      301 gactccatga aagacgagga gtacgagcag atggtcaagg agctgcagga gatccgcctc
      361 gagccgcagg aggtgccgcg cggctcggag ccctggaagc agcagctcac cgaggacggg
      421 gactcgttcc tgcacttggc catcatccat gaagaaaagg cactgaccat ggaagtgatc
      481 cgccaggtga agggagacct ggccttcctc aacttccaga acaacctgca gcagactcca
      541 ctccacttgg ctgtgatcac caaccagcca gaaattgctg aggcacttct gggagctggc
      601 tgtgatcctg agctccgaga ctttcgagga aatacccccc tacaccttgc ctgtgagcag
      661 ggctgcctgg ccagcgtggg agtcctgact cagtcctgca ccaccccgca cctccactcc
      721 atcctgaagg ctaccaacta caatggccac acgtgtctac acttagcctc tatccatggc
      781 tacctgggca tcgtggagct tttggtgtcc ttgggtgctg atgtcaatgc tca
//
