LOCUS       sequence-64678-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lacritin-intein fusion plasmid coding for intact mature lacritin 
            without signal peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Wang J, Wang N, Xie J, Walton SC, McKown RL, Raab RW, Ma P, Beck SL,
            Coffman GL, Hussaini IM, Laurie GW
  TITLE     Restricted epithelial proliferation by lacritin via 
            PKCalpha-dependent NFAT and mTOR pathways.
  JOURNAL   J Cell Biol. 2006 Aug 28;174(5):689-700. Epub 2006 Aug 21.
  PUBMED    16923831
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-08-28"; volume: "174(5)"; pages: "689-700. Epub 
            2006 Aug 21"
COMMENT     Sequence Label: pLAC
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..188)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             complement(298..309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttgttagcag ccggatcccc ttcctgcagt cattgaagct gccacaaggc aggaacgttg
       61 gatggttccc atcctgccaa ggaggtgtgg ggctgcaaac atttatacgt cttgccgtta
      121 tatgtgacca attgtcccgc agtataagct gtgttgacct gccaagcgga tacaccagga
      181 tttgtcgtga ggcctgagtt cagaccggtc aggccaccgc atgcatggac gacaacctgg
      241 gatccaagca aaaactgatg atcagaatca tcagataaag taatcccata ataatcgtct
      301 tccttcaatt cttgtaactc gaaataaaat ccgcggcact cacgtgcaaa agcagcggcg
      361 ggagcaggcc tgaatttttt agagccggca cacttcgaaa gaacgttaag caaaacatct
      421 ccaccagaca tataaatagc ataactaatt ttatgtttgg tgacattcat gtcaacctta
      481 gcaggttctg cgttaaccga gactactaag cctaaagaac gagcaaggga aaccaaacca
      541 tctctgacag aagtatgaat tgtctttatt gttgctttaa taccatgctc atcagtaaca
      601 tagccatcag aatcaattag accagcaaga aatgtttcac gagtaccgat attgtccgta
      661 gacaagaaag aaggaatatt tttgacaccg tccttcaaga atcctaagcc aacaatagcg
      721 tcccataatg gattctcagt attaagatta ttgcgaatac cattacctct gacaacttta
      781 gagtacaaat taacagtttt ggcaacttgt ggttcttttc tgtccttata ctcggcgcac
      841 aaattcaact tttcagcata ttcagtaaca cgttc
//
