LOCUS       sequence-64679-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lacritin-intein fusion plasmid coding for truncated lacritin without
            signal peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Wang J, Wang N, Xie J, Walton SC, McKown RL, Raab RW, Ma P, Beck SL,
            Coffman GL, Hussaini IM, Laurie GW
  TITLE     Restricted epithelial proliferation by lacritin via 
            PKCalpha-dependent NFAT and mTOR pathways.
  JOURNAL   J Cell Biol. 2006 Aug 28;174(5):689-700. Epub 2006 Aug 21.
  PUBMED    16923831
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-08-28"; volume: "174(5)"; pages: "689-700. Epub 
            2006 Aug 21"
COMMENT     Sequence Label: C25-pLAC
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(332..880)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="modified 3' region of the intein from the yeast 
                     Vma1 subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 3' region
                     /note="C-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="GHGGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRE
                     TFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNVT
                     KHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGI
                     TLSDDSDHQFLLGSQVVVQN"
ORIGIN
        1 tagcagccgg atccccttcc tgcagtcacc cgggctcgag gaattctcat ccacctggca
       61 cgcctccgtg cattcctttt cctgcttttg caagggcttg ttctgttagt aagatacttt
      121 tctccactat ggatttcagg gggtttagtt cctgcctgct tgaggtgacc ttggctgtcc
      181 cctgaactgc tgccgccgaa gtctcctggg ctgttgtggt tgtctctggg ggtgaagctg
      241 gttctgctgg acctgagatc tcttcattag gcttagaggt cccagcttcc tgggcaggat
      301 cagcacccgt cgagtcagag gaggcatctt cgttctgtac aacaacctgg gatccaagca
      361 aaaactgatg atcagaatca tcagataaag taatcccata ataatcgtct tccttcaatt
      421 cttgtaactc gaaataaaat ccgcggcact cacgtgcaaa agcagcggcg ggagcaggcc
      481 tgaatttttt agagccggca cacttcgaaa gaacgttaag caaaacatct ccaccagaca
      541 tataaatagc ataactaatt ttatgtttgg tgacattcat gtcaacctta gcaggttctg
      601 cgttaaccga gactactaag cctaaagaac gagcaaggga aaccaaacca tctctgacag
      661 aagtatgaat tgtctttatt gttgctttaa taccatgctc atcagtaaca tagccatcag
      721 aatcaattag accagcaaga aatgtttcac gagtaccgat attgtccgta gacaagaaag
      781 aaggaatatt tttgacaccg tccttcaaga atcctaagcc aacaatagcg tcccataatg
      841 gattctcagt attaagatta ttgcgaatac caccatggcc accttga
//
