LOCUS       sequence-64681-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Shcherbakova DM, Verkhusha VV
  TITLE     Near-infrared fluorescent proteins for multicolor in vivo imaging.
  JOURNAL   Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.
  PUBMED    23770755
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521."
COMMENT     Sequence Label: pBAD/HisB-iRFP670
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..105
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             109..141
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(145..153,154..168)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt tttgggctaa
       61 caggaggaat taaccatggg gggttctcat catcatcatc atcatggtat ggctagcatg
      121 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tccgagctcg
      181 agatctatgc cgcgtaaggt cgatctcacc tcctgcgatc gcgagccgat ccacatcccc
      241 ggcagcattc agccgtgcgg ctgcctgcta gcctgcgacg cgcaggcggt gcggatcacg
      301 cgcattacgg aaaatgccgg cgcgttcttt ggacgcgaaa ctccgcgggt cggtgagcta
      361 ctcgccgatt acttcggcga gaccgaagcc catgcgctgc gcaacgcact ggcgcagtcc
      421 tccgatccaa agcgaccggc gctgatcttc ggttggcgcg acggcctgac cggccgcacc
      481 ttcgacatct cactgcatcg ccatgacggt acatcgatca tcgagttcga gcctgcggcg
      541 gccgaacagg ccgacaatcc gctgcggctg acgcggcaga tcatcgcgcg caccaaagaa
      601 ctgaagtcgc tcgaagagat ggccgcacgg gtgccgcgct atctgcaggc gatgctcggc
      661 tatcaccgcg tgatgttgta ccgcttcgcg gacgacggct ccgggatggt gatcggcgag
      721 gcgaagcgca gcgacctcga gagctttctc ggtcagcact ttccggcgtc gctggtcccg
      781 cagcaggcgc ggctactgta cttgaagaac gcgatccgcg tggtctcgga ttcgcgcggc
      841 atcagcagcc ggatcgtgcc cgagcacgac gcctccggcg ccgcgctcga tctgtcgttc
      901 gcgcacctgc gcagcatctc gccctgccat ctcgaatttc tgcgg
//
