LOCUS       sequence-64690-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Shcherbakova DM, Verkhusha VV
  TITLE     Near-infrared fluorescent proteins for multicolor in vivo imaging.
  JOURNAL   Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.
  PUBMED    23770755
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521."
COMMENT     Sequence Label: pBAD/HisB-iRFP682
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..105
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             109..141
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(145..153,154..168)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt tttgggctaa
       61 caggaggaat taaccatggg gggttctcat catcatcatc atcatggtat ggctagcatg
      121 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tccgagctcg
      181 agatctatgg cggaaggatc cgtcgccagg cagcctgacc tcttgacctg cgacgatgag
      241 ccgatccata tccccggtgc catccaaccg catggactgc tgctcgccct cgccgccgac
      301 atgacgatcg ttgccggcag cgacaacctt cccgaactca ccggactggc gatcggcgcc
      361 ctgatcggcc gctctgcggc cgatgtcttc gactcggaga cgcacaaccg tctgacgatc
      421 gccttggccg agcccggggc ggccgtcgga gcaccgatca ctgtcggctt cacgatgcga
      481 aaggacgcag gcttcatcgg ctcctggcat cgccatgatc agctcatctt cctcgagctc
      541 gagcctcccc agcgggacgt cgccgagccg caggcgttct tccgccgcac caacagcgcc
      601 atccgccgcc tgcaggccgc cgaaaccttg gaaagcgcct gcgccgccgc ggcgcaagag
      661 gtgcggaaga ttaccggctt cgatcgggtg atgatctatc gcttcgcctc cgacttcagc
      721 ggggtggtga tcgcagagga tcgatgcgcc gaggtcgagt caaaactagg cctgcactat
      781 cctgcctcag cggtgccggc gcaggcccgt cggctctata ccatcaaccc ggtacggatc
      841 attcccgata tcaattatcg gccggtgccg gtcaccccag acctcaatcc ggtcaccggg
      901 cggccgattg atcttagctt cgccatcctg
//
