LOCUS       sequence-64695-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Han C, Jan LY, Jan YN
  TITLE     Enhancer-driven membrane markers for analysis of nonautonomous 
            mechanisms reveal neuron-glia interactions in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9673-8. doi: 
            10.1073/pnas.1106386108. Epub 2011 May 23.
  PUBMED    21606367
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1106386108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-06-7- 7"; volume: "108"; issue: "23"; pages: "9673-8"
COMMENT     Sequence Label: pDEST-HemmarR
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(140..264)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    298..683
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      241 ttcagctttt ttgtacaaac ttgtgatgat ctgctgcagc atgctgttgc cgagcacaat
      301 tgatcggcta aatggtatgg caagaaaagg tatgcaatat aataatcttt tattgggtat
      361 gcaacgaaaa tttgtttcgt caacgtatgc aatatttttt attaaaagag ggtatgcaat
      421 gtattttatt aaaaacgggt atgcaatata ataatctttt attgggtatg caacgaaaat
      481 ttgtttcgtc aaagtatgca atatttttta ttaaaagagg gtatgcaatg tattttatta
      541 aaaacgggta tgcaataaaa aattatttgg tttctctaaa aagtatgcag cacttatttt
      601 ttgataaggt atgcaacaaa attttacttt gccgaaaata tgcaatgttt ttgcgaataa
      661 attcaacgca cacttattac gtgatgcagc caagcttggc gaacagttcg gctggcgcga
      721 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
      781 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
      841 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcag
//
