LOCUS       sequence-64717-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCU-HHT2
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(204..419)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(443..462)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     562..584
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(622..640)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca
       61 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc
      121 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt
      181 tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc
      241 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat
      301 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
      361 aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa
      421 aggtggtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
      481 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      541 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
      601 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      661 taataatggt ttcttagcag gttagactaa catgcaaaaa gtttatattc atttttgtag
      721 ttattcttgt tttcattagg atgattacac actgtggatg tggattaatc gatcattgtt
      781 aatttagtgt agctactacc atttcaaaca aaacgtaaat tcttgcagtc gtactggatc
      841 tgtgaatcta ttagtatata tgaattaaag tagcttg
//
