LOCUS       sequence-64723-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Shcherbakova DM, Verkhusha VV
  TITLE     Near-infrared fluorescent proteins for multicolor in vivo imaging.
  JOURNAL   Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.
  PUBMED    23770755
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521."
COMMENT     Sequence Label: piRFP682-N1
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    32..112
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
      121 tggcggaagg atccgtcgcc aggcagcctg acctcttgac ctgcgacgat gagccgatcc
      181 atatccccgg tgccatccaa ccgcatggac tgctgctcgc cctcgccgcc gacatgacga
      241 tcgttgccgg cagcgacaac cttcccgaac tcaccggact ggcgatcggc gccctgatcg
      301 gccgctctgc ggccgatgtc ttcgactcgg agacgcacaa ccgtctgacg atcgccttgg
      361 ccgagcccgg ggcggccgtc ggagcaccga tcactgtcgg cttcacgatg cgaaaggacg
      421 caggcttcat cggctcctgg catcgccatg atcagctcat cttcctcgag ctcgagcctc
      481 cccagcggga cgtcgccgag ccgcaggcgt tcttccgccg caccaacagc gccatccgcc
      541 gcctgcaggc cgccgaaacc ttggaaagcg cctgcgccgc cgcggcgcaa gaggtgcgga
      601 agattaccgg cttcgatcgg gtgatgatct atcgcttcgc ctccgacttc agcggggtgg
      661 tgatcgcaga ggatcgatgc gccgaggtcg agtcaaaact aggcctgcac tatcctgcct
      721 cagcggtgcc ggcgcaggcc cgtcggctct ataccatcaa cccggtacgg atcattcccg
      781 atatcaatta tcggccggtg ccggtcaccc cagacctcaa tccggtcacc gggcggccga
      841 ttgatcttag cttcgccatc ctgcgcagcg tctcgccctg ccatttggag ttcatgcgca
      901 acataggcat gcacggcacg atgtcgatct cgattttgcg cggcgagcga ctgtggggat
      961 tgatcgtttg ccatcaccga acgccgtact acgtcgatct cgatggccgc caagcctgcg
     1021 agctagtc
//
