LOCUS       sequence-64728-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCN-LYS21
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..569)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPC"
ORIGIN
        1 gggcagggca tgctcatgta gagcgcctgc tcgccgtccg aggcggtgcc gtcgtacagg
       61 gcggtgtcca ggccgcagag ggtgaacccc atccgccggt acgcgtggat cgccggtgcg
      121 ttgacgttgg tgacctccag ccagaggtgc ccggcgccca gctcgcgggc gaactccgtc
      181 gcgagcccca tcaacgcgcg cccgaccccg tgcccccggt gctccggggc gacctcgatg
      241 tcctcgacgg tcagccggcg gttccagccg gagtacgaga cgaccacgaa gcccgccagg
      301 tcgccgtcgt ccccgtacgc gacgaacgtc cgggagtcag ggtcgccgtc ctccccgtcg
      361 tccgattcgt cgtccgattc gtcgtcgggg aacaccttgg tcaggggcgg gtccaccggc
      421 acctcccgca gggtgaagcc gtccccggtg gcggtgacgc ggaagacggt gtcggtggtg
      481 aaggacccat ccagtgcctc gatggcctcg gcgtcccccg ggacactggt gcggtatcgg
      541 taagccgtgt cgtcaagagt ggtgcccatt ttagatccaa aacttagatt agattgctat
      601 gctttctttc taatgagcaa gaagtaaaaa aagttgtaat agaacaagaa aaatgaaact
      661 gaaacttgag aaattgaaga ccgtttatta acttaaatat caatgggagg tcatcgaaag
      721 agaaaaaaat caaaaaaaaa attttcaaga aaaagaaacg tgataaaaat ttttattgcc
      781 tttttcgacg aagaaaaaga aacgaggcgg tctctttttt cttttccaaa cctttagtac
      841 gggtaattaa cgacacccta gaggaagaaa gaggggaaat ttagtatgct gtgcttgggt
      901 gttttgaagt g
//
