LOCUS       sequence-64732-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pGRB2.1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     428..444
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     429..445
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(479..495)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(479..495)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(532..552)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(532..550)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(571..587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(571..587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(584..606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    595..611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(619..625,626..643,644..649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    664..685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(802..819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tgggtacctt tacctctata tcgtgttcgt gcgtatttct
       61 tcctctataa tatgaaaaga aaggcagtag tcttgatttt ctattcttcc aaatattaca
      121 agaataccca gaaccacaag aagcagaaga aactaactgc tttatatact taatttcccg
      181 ccacaagctc atcgcccaga agtttttttc agtttttcac ctgacaatct ggcagctcgc
      241 tgaaaaattt tcacctgggg ttgcctgcta cgtaaagtga tcacgtttat gaatggcaca
      301 ttatatagtg gcccagacgg tctgatggta gttcatgtag ttaagaataa atactctgtt
      361 tgtgtgattt ctatgtgtac gttatatata taaatattgt tgatggtggt agctgtgggt
      421 tgtgttctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
      481 tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
      541 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      601 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      661 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      721 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      781 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      841 gcgagcggta tcagctcact caa
//
