LOCUS       sequence-64735-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pBM16
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(57..74)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(57..73)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(66..88)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(273..520)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     486..504
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc gaattcactg
       61 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      121 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      181 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
      241 catctgtgcg gtatttcaca ccgcatacgg ccgcaaatta aagccttcga gcgtcccaaa
      301 accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgtct gtacagaaaa
      361 aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat aaaaaaataa
      421 atagggacct agacttcagg ttgtctaact ccttcctttt cggttagagc ggatgtgggg
      481 ggagggcgtg aatgtaagcg tgacataact aattacatga ctcgacttag gggcagggca
      541 tgctcatgta gagcgcctgc tcgccgtccg aggcggtgcc gtcgtacagg gcggtgtcca
      601 ggccgcagag ggtgaacccc atccgccggt acgcgtggat cgccggtgcg ttgacgttgg
      661 tgacctccag ccagaggtgc ccggcgccca gctcgcgggc gaactccgtc gcgagcccca
      721 tcaacgcgcg cccgaccccg tgcccccggt gctccggggc gacctcgatg tcctcgacgg
      781 tcagccggcg gttccagccg gagtacgaga cgaccacgaa gcccgccagg tcgccgtcgt
      841 ccccgtacgc gacgaacgtc cgggagtcag ggtcgccgtc ctccccgtcg tccgattcgt
      901 cgtc
//
