LOCUS       sequence-64737-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   de la Fuente S, Fonteriz RI, de la Cruz PJ, Montero M, Alvarez J
  TITLE     Mitochondrial free [Ca(2+)] dynamics measured with a novel 
            low-Ca(2+) affinity aequorin probe.
  JOURNAL   Biochem J. 2012 Aug 1;445(3):371-6. doi: 10.1042/BJ20120423.
  PUBMED    22671130
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2012-08-1- 1"; volume: "445"; issue: "3"; pages:
            "371-6"
COMMENT     Sequence Label: pcDNA3.1+/mit-2mutAEQ
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             148..222
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             253..279
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc gggtactccg tgccatcatg tccgtcctga cgccgctgct gctgcggggc
      181 ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca agatccattc gttgccgccg
      241 gaggggaagc tttatgatgt tcctgattat gctagcctca aacttacatc agacttcgac
      301 aacccaagat ggattggacg acacaagcat atgttcaatt tccttgatgt cgaccacctt
      361 ggaaaaatct ctcttgacga gatggtctac aaggcatctg atattgtcat caataacctt
      421 ggagcaacac ctgagcaagc caaacgacac aaagatgctg tagaagcctt cttcggagga
      481 gctggaatga aatatggtgt ggaaactgat tggcctgcat atattgaagg atggaaaaaa
      541 ttggctactg atgaattgga gaaatacgcc aaaaacgaac caacgctcat ccgtatatgg
      601 ggtgatgctt tgtttgatat cgtcgacaaa gcccaaaatg gagccattac actggatgaa
      661 tggaaagcat acaccaaagc tgctggtatc atccaatcat cagaagattg cgaggaaaca
      721 ttcagagtgt gcgatattga tgaaagtgga caactcgatg ttgatgagat gacaagacaa
      781 catttaggat tttggtacac catggatcct gcttgcgaaa agctctacgg tggagctgtc
      841 ccctaagaag ctctacggtg gtgatgcacc ctaggaagat gatgtgattt tgaataaaac
      901 actgatgaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
      961 taa
//
