LOCUS       sequence-64739-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   de la Fuente S, Fonteriz RI, de la Cruz PJ, Montero M, Alvarez J
  TITLE     Mitochondrial free [Ca(2+)] dynamics measured with a novel 
            low-Ca(2+) affinity aequorin probe.
  JOURNAL   Biochem J. 2012 Aug 1;445(3):371-6. doi: 10.1042/BJ20120423.
  PUBMED    22671130
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2012-08-1- 1"; volume: "445"; issue: "3"; pages:
            "371-6"
COMMENT     Sequence Label: pcDNA3.1+/ER-2mutAEQ
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     join(157..201,284..295)
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGVHS"
     CDS             join(157..202,285..295)
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGVHS"
     primer_bind     267..286
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc gagctcggta ccctgtcgac ctcaccatgg gatggagctg tatcatcctc
      181 ttcttggtag caacagctac aggtaagggg ctcacagtag caggcttgag gtctggacat
      241 atatatgggt gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt
      301 ccaactgcag cagcctgggg ctgagcttgt gaagcctggg gcttcagtga agctgtcctg
      361 caaggcttct ggctacacct tcaccagcta ctggatgcac tgggtgaagc agaggcctgg
      421 acgaggcctt gagtggattg gaaggattga tcctaatagt ggtggtacta agtacaatga
      481 gaagttcaag agcaaggcca cactgactgt agacaaaccc tccagcacag cctacatgca
      541 gctcagcagc ctgacatctg aggactctgc ggtctattat tgtgcaagat acgattacta
      601 cggtagtagc tactttgact actggggcca aggcaccact ctcacagtct cctcaggtga
      661 gtccttacaa cctctctctt ctattcagct taaatagatt ttactgcatt tgttgggggg
      721 gaaatgtgtg tatctgaatt tcaggtcatg aaggactagg gacaccttgg gagtcagaaa
      781 gggtcattgg gagccctggc tgatgcagac agacatcctc agctcccaga cttcatggcc
      841 agagatttat agggatctct agaagaatgc agcatacact cctatttcaa ggggtactg
//
