LOCUS       sequence-64742-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Arlotta P, Molyneaux BJ, Chen J, Inoue J, Kominami R, Macklis JD
  TITLE     Neuronal subtype-specific genes that control corticospinal motor 
            neuron development in vivo.
  JOURNAL   Neuron. 2005 Jan 20;45(2):207-21.
  PUBMED    15664173
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2005-01-20"; volume: "45(2)"; pages: "207-21"
COMMENT     Sequence Label: pCRII-Topo Crym in situ probe
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(608..627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(609..627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(634..651)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(634..650)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(643..665)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(878..897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttactggcga gaactggatg
      121 acgagctcat gaggcaagcg gtgctgtatg tggactcccg ggaggctgcc ctgaaggagt
      181 caggagacgt tctgttgtca ggggctgaca tctttgctga gcttggagaa gtgatttcag
      241 gagcgaagcc tgcacactgt gagaagacca cagtgttcaa atctttgggg atggcagtgg
      301 aagacctggt tgcagccaaa ttagtatatg attcttggtc atctggcaag tgagttgaag
      361 gaaccgtgcc tgagttggcc atcacagctc aacactgttt cacaagtgtc aaaatcaaag
      421 gaggtccagt ccccagtgaa tggtagtgat tgtcattcat aagtactgac acccctattc
      481 atgtttgtgg ttggatagct aaaccaggta accatttctt ctgttaaggg gtgatggcaa
      541 gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc
      601 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
      661 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      721 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      781 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      901 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggg
//
