LOCUS       sequence-64745-e       10191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA
  TITLE     Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension
            across VE-Cadherin and PECAM-1.
  JOURNAL   Curr Biol. 2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049.
  PUBMED    23684974
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049."
COMMENT     Sequence Label: pLPCX-VEcadTL
FEATURES             Location/Qualifiers
     source          1..10191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1566..2165
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(1566..1585)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2062..2082
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2340..2643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2644..2847
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2798..2818
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4926..5633
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(5754..5756,5757..5759,5760..6473)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5799..5820)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6060..6079)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6407..6428
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6930..7523
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7626..7648)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    7776..7809
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7869..7902
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7922..7941
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8022..8039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8193..8781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8273..8292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8952..9743,9744..9812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9575..9594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9813..9917)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9985..10003
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
     2881 cggactcaga tctcgagctc aagcttgata tcaccatgca gaggctcaca gagctggcca
     2941 ctgctttggg agccttcctg ggcctcctgg cagtggcagc aatggcaggc cctaactttc
     3001 cccagataga cacccccaac atgctacctg cccaccatcg ccaaaagaga gactggattt
     3061 ggaatcaaat gcacatcgat gaagagaaaa atgaatcgct gccccactat gtgggaaaga
     3121 tcaagtccaa cgtgaaccgc cagaatgcta agtatgtgct ccaaggagag tttgctggca
     3181 agatcttcgg ggtcgatgct aacacaggga atgtgcttgc ctatgagagg ctagaccggg
     3241 agaaagtctc tgagtacttc cttactgccc tcattgtgga caagaacacc aacaaaaacc
     3301 tggaacaacc ttccagcttc actgtcaagg tgcatgatat aaatgacaat tggcctgtgt
     3361 tttcgcacca ggtattcaac gcatctgtgc cagagatgtc agctataggg acctctgtca
     3421 tccgtgtgac agcagtggat gcagatgacc ccactgtggc cggccacgcc actgtcttgt
     3481 accaaatcgt gaaaggaaat gagtatttca gcattgataa ttctggactc attttcacaa
     3541 agatcaaaaa cttggaccga gagaaacagg ctgaatacaa gatcgtggtg gaaacacaag
     3601 atgccctggg tctgcgggga gagtcaggca cagccactgt gatgatcaga ctggaggaca
     3661 tcaatgacaa cttccccgtc tttactcaat ccacatacac attttcggtg cctgaagaca
     3721 tccgagtggg caagcccttg ggctttctga ctgttgtgga cccagatgag cctcagaacc
     3781 ggatgaccaa gtacagcatc atgcagggcg agtacaggga caccttcacc attgagacag
     3841 accccaaacg taacgagggt atcatcaaac ccacgaagtc cctggactat gaagtcatcc
     3901 agcagtacac attctacatt gaggccacag accccactat ccgatacgaa tacctgagca
     3961 gcacttcagg caaaaacaaa gccatggtca ccatcaacgt cctagatgtg gatgagcccc
     4021 ctgtcttcca gcgacacttc taccacttca agctgccaga aaaccagaag aaaccgctga
     4081 tcggcactgt ggtggccaaa gaccctgaca aggctcagcg cagcatcggg tactccatcc
     4141 gcaagaccag tgacagaggc caattcttcc gaataaccaa gcagggaaac atctataacg
     4201 agaaagaact ggacagagaa acctacgcct ggtataacct gactgtggag gccaacgaac
     4261 tggattctcg gggtaaccct gtagggaaag agtccattgt gcaggtttac attgaagtct
     4321 tggatgagaa tgacaaccct ccggaatttg cccagcccta cgaacctaaa gtgtgtgaga
     4381 atgctgccca gggcaagctg gtagtacaga tctctgcaac agacaaggat gtggtgccag
     4441 taaacccaaa gttcaagttt gccctgaaga acgaggacag caacttcacc ctcataaaca
     4501 accatgacaa caccgccaac atcacggtca agtatgggca gtttaatcgg gagcatgcca
     4561 agttccacta cctgcccgtg ctcatctcag acaacggggt gcccagcctg actggaacca
     4621 gcacgctaac cgtgggtgtg tgcaagtgca acgagcaggg cgagttcacc ttctgtgagg
     4681 agatggcagc ccaggcgggt gtcagcatcc aggcgctggt ggccatcttc ctctgcatcc
     4741 tcaccatcac agtgattacc ttgctgatca tcctgcggag gcggatccgg aagcaggcgc
     4801 atgctcatag caagagtgcg ctggagattc acgagcagtt ggtcacttac gatgaggagg
     4861 gcggtggtga gatggacacc accagctatg atgtgtcagt gcttaattct gtgcgcggtg
     4921 tcgagatggt gagcaagggc gaggagacca caatgggcgt aatcaagccc gacatgaaga
     4981 tcaagctgaa gatggagggc aacgtgaatg gccacgcctt cgtgatcgag ggcgagggcg
     5041 agggcaagcc ctacgacggc accaacacca tcaacctgga ggtgaaggag ggagcccccc
     5101 tgcccttctc ctacgacatt ctgaccaccg cgttcgccta cggcaacagg gccttcacca
     5161 agtaccccga cgacatcccc aactacttca agcagtcctt ccccgagggc tactcttggg
     5221 agcgcaccat gaccttcgag gacaagggca tcgtgaaggt gaagtccgac atctccatgg
     5281 aggaggactc cttcatctac gagatacacc tcaagggcga gaacttcccc cccaacggcc
     5341 ccgtgatgca gaagaagacc accggctggg acgcctccac cgagaggatg tacgtgcgcg
     5401 acggcgtgct gaagggcgac gtcaagcaca agctgctgct ggagggcggc ggccaccacc
     5461 gcgttgactt caagaccatc tacagggcca agaaggcggt gaagctgccc gactatcact
     5521 ttgtggacca ccgcatcgag atcctgaacc acgacaagga ctacaacaag gtgaccgttt
     5581 acgagagcgc cgtggcccgc aactccaccg acggcatgga cgagctgtac aaggggccag
     5641 gtggtgcagg gccaggtggt gcagggccag gtggtgcagg gccaggtggt gcagggcccg
     5701 gtggtgcagg tccaggtggt gcaggtccag gtggtgcagg tccaggtggt gctatggtga
     5761 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5821 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     5881 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5941 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     6001 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     6061 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     6121 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6181 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     6241 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     6301 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6361 gctaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6421 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagcggccg
     6481 cgggctccac taagcccctg cgctccacga tggacgcccg gccggcagtg tacacgcagg
     6541 tgcagaagcc gccgcgcctg gcgcctggac tgcacggagg gcccagggag atggccacca
     6601 tgatcgacgt gaagaaagaa gaggcagaca atgacggcgg tggcccccca tacgacacac
     6661 tgcacatcta cggctacgag ggcgcagagt ccatcgcaga gtccctcagt tccctgagca
     6721 ccaattcctc cgactctgac atcgattatg acttcctcaa cgactgggga cccaggttca
     6781 agatgctggc tgagctgtac ggctcagatc cccaggagga actcatcatc taggaattcg
     6841 ttaggccatt aaggcctgtc gacaagcggc cgcctcggcc aaacatcgat aaaataaaag
     6901 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     6961 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     7021 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     7081 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     7141 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     7201 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     7261 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     7321 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     7381 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     7441 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     7501 cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg
     7561 gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg tgatgacggt
     7621 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     7681 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     7741 atgacccagt cacgtagcga tagcggagtg tactgataac ttcgtataat gtatgctata
     7801 cgaagttatt aggtctgaag aggagtttac gtccagccaa gctagggccg cgatccggaa
     7861 cccttaatat aacttcgtat aatgtatgct atacgaagtt atcagtactg gcttaactat
     7921 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     7981 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     8041 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8101 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8161 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8221 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8281 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8341 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8401 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8461 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8521 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8581 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8641 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8701 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8761 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8821 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9241 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     9301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9541 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     9601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9961 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
    10021 ttgctagcaa ttgctagcaa ttgctagcaa ttcataccag atcaccgaaa actgtcctcc
    10081 aaatgtgtcc ccctcacact cccaaattcg cgggcttctg cctcttagac cactctaccc
    10141 tattccccac actcaccgga gccaaagccg cggcccttcc gtttctttgc t
//
