LOCUS       sequence-64753-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Molyneaux BJ, Arlotta P, Hirata T, Hibi M, Macklis JD
  TITLE     Fezl is required for the birth and specification of corticospinal 
            motor neurons.
  JOURNAL   Neuron. 2005 Sep 15;47(6):817-31.
  PUBMED    16157277
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2005-09-15"; volume: "47(6)"; pages: "817-31"
COMMENT     Sequence Label: pCRII-Topo Plexin D1 in situ probe
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(828..847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(829..847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(854..871)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(854..870)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(863..885)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc tttgagggac acagacaact
      121 gcttggtggc tgaagcagaa attccctctg ccaggtggat gaggtagtgt tctgggatat
      181 ggccacctct cccaatgtgt gctgagcagc ctgaaacagt gaacaggcag gcatggtcca
      241 ggctgtcctg gttggcaggg aaagtcttat tagggactgg cagcgatcga tattcaaggc
      301 cttcatccta gagttgggtc ttgggcaggt ggaggggaca gagatgcccc aggggacaag
      361 gccaagagct cggcagccgg gcactaagag gccagtgtcc cccactccta gcccagcagg
      421 ctcctctgtc agttcccagc ccaagtgaga gatgtgggga agaaaccaag tggaggccaa
      481 gtggcctgca cgccgtctcc ctggccgaag ctcctggtca ctcttctgca tcaggcctcg
      541 ctgtaacact catagatatt gttttccatc agagccacca cctgttcaaa cttgtgctgt
      601 agctgggtcc tgcgggctgt ggggttggcc tccagggcag ccatgatctg tggtcgatac
      661 ctcttagcat atttataaat ctcagccatg gccacgtttg tgttgaactc attctggtat
      721 ttccgagact cctcggccag gtgtgcgttc atttcctgaa gggcgaattc tgcagatatc
      781 catcacactg gcggccgctc gagcatgcat ctagagggcc caattcgccc tatagtgagt
      841 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      901 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      961 cccgcaccga tcgcccttcc caacagttgc gca
//
