LOCUS       sequence-64754-e       10047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA
  TITLE     Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension
            across VE-Cadherin and PECAM-1.
  JOURNAL   Curr Biol. 2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049.
  PUBMED    23684974
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049."
COMMENT     Sequence Label: pLPCX-PECAMTL
FEATURES             Location/Qualifiers
     source          1..10047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1566..2165
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(1566..1585)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2062..2082
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2340..2643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2644..2847
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2798..2818
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             5073..5780
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(5901..5903,5904..5906,5907..6620)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5946..5967)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6207..6226)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6554..6575
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6786..7379
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7482..7504)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    7632..7665
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7725..7758
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7778..7797
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7878..7895)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8049..8637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8129..8148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8808..9599,9600..9668))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9431..9450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9669..9773)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9841..9859
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
     2881 cggactcaga tctcgagctc aagcttagga tgcagccgag gtgggcccaa ggggccacga
     2941 tgtggcttgg agtcctgctg acccttctgc tctgttcaag ccttgagggt caagaaaact
     3001 ctttcacaat caacagtgtt gacatgaaga gcctgccgga ctggacggtg caaaatggga
     3061 agaacctgac cctgcagtgc ttcgcggatg tcagcaccac ctctcacgtc aagcctcagc
     3121 accagatgct gttctataag gatgacgtgc tgttttacaa catctcctcc atgaagagca
     3181 cagagagtta ttttattcct gaagtccgga tctatgactc agggacatat aaatgtactg
     3241 tgattgtgaa caacaaagag aaaaccactg cagagtacca gctgttggtg gaaggagtgc
     3301 ccagtcccag ggtgacactg gacaagaaag aggccatcca aggtgggatc gtgagggtca
     3361 actgttctgt cccagaggaa aaggccccaa tacacttcac aattgaaaaa cttgaactaa
     3421 atgaaaaaat ggtcaagctg aaaagagaga agaactctcg agaccagaat tttgtgatac
     3481 tggagttccc cgttgaggaa caggaccgcg ttttatcctt ccgatgtcaa gctaggatca
     3541 tttctgggat ccatatgcag acctcagaat ctaccaagag tgaactggtc accgtgacgg
     3601 aatccttctc tacacccaag ttccacatca gccccaccgg aatgatcatg gaaggagctc
     3661 agctccacat taagtgcacc attcaagtga ctcacctggc ccaggagttt ccagaaatca
     3721 taattcagaa ggacaaggcg attgtggccc acaacagaca tggcaacaag gctgtgtact
     3781 cagtcatggc catggtggag cacagtggca actacacgtg caaagtggag tccagccgca
     3841 tatccaaggt cagcagcatc gtggtcaaca taacagaact attttccaag cccgaactgg
     3901 aatcttcctt cacacatctg gaccaaggtg aaagactgaa cctgtcctgc tccatcccag
     3961 gagcacctcc agccaacttc accatccaga aggaagatac gattgtgtca cagactcaag
     4021 atttcaccaa gatagcctca aagtcggaca gtgggacgta tatctgcact gcaggtattg
     4081 acaaagtggt caagaaaagc aacacagtcc agatagtcgt atgtgaaatg ctctcccagc
     4141 ccaggatttc ttatgatgcc cagtttgagg tcataaaagg acagaccatc gaagtccgtt
     4201 gcgaatcgat cagtggaact ttgcctattt cttaccaact tttaaaaaca agtaaagttt
     4261 tggagaatag taccaagaac tcaaatgatc ctgcggtatt caaagacaac cccactgaag
     4321 acgtcgaata ccagtgtgtt gcagataatt gccattccca tgccaaaatg ttaagtgagg
     4381 ttctgagggt gaaggtgata gccccggtgg atgaggtcca gatttctatc ctgtcaagta
     4441 aggtggtgga gtctggagag gacattgtgc tgcaatgtgc tgtgaatgaa ggatctggtc
     4501 ccatcaccta taagttttac agagaaaaag agggcaaacc cttctatcaa atgacctcaa
     4561 atgccaccca ggcattttgg accaagcaga aggctagcaa ggaacaggag ggagagtatt
     4621 actgcacagc cttcaacaga gccaaccacg cctccagtgt ccccagaagc aaaatactga
     4681 cagtcagagt cattcttgcc ccatggaaga aaggacttat tgcagtggtt atcatcggag
     4741 tgatcattgc tctcttgatc attgcggcca aatgttattt tctgaggaaa gccaaggcca
     4801 agcagatgcc agtggaaatg tccaggccag cagtaccact tctgaactcc aacaacgaga
     4861 aaatgtcaga tcccaatatg gaagctaaca gtcattacgg tcacaatgac gatgtcagaa
     4921 accatgcaat gaaaccaata aatgataata aagagcctct gaactcagac gtgcagtaca
     4981 cggaagttca agtgtcctca gctgagtctc acaaagatct aggaaagaag gacacagaga
     5041 cagtgtacag tgaagtccgg aaagctgtcg agatggtgag caagggcgag gagaccacaa
     5101 tgggcgtaat caagcccgac atgaagatca agctgaagat ggagggcaac gtgaatggcc
     5161 acgccttcgt gatcgagggc gagggcgagg gcaagcccta cgacggcacc aacaccatca
     5221 acctggaggt gaaggaggga gcccccctgc ccttctccta cgacattctg accaccgcgt
     5281 tcgcctacgg caacagggcc ttcaccaagt accccgacga catccccaac tacttcaagc
     5341 agtccttccc cgagggctac tcttgggagc gcaccatgac cttcgaggac aagggcatcg
     5401 tgaaggtgaa gtccgacatc tccatggagg aggactcctt catctacgag atacacctca
     5461 agggcgagaa cttccccccc aacggccccg tgatgcagaa gaagaccacc ggctgggacg
     5521 cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc aagcacaagc
     5581 tgctgctgga gggcggcggc caccaccgcg ttgacttcaa gaccatctac agggccaaga
     5641 aggcggtgaa gctgcccgac tatcactttg tggaccaccg catcgagatc ctgaaccacg
     5701 acaaggacta caacaaggtg accgtttacg agagcgccgt ggcccgcaac tccaccgacg
     5761 gcatggacga gctgtacaag gggccaggtg gtgcagggcc aggtggtgca gggccaggtg
     5821 gtgcagggcc aggtggtgca gggcccggtg gtgcaggtcc aggtggtgca ggtccaggtg
     5881 gtgcaggtcc aggtggtgct atggtgagca agggcgagga gctgttcacc ggggtggtgc
     5941 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     6001 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc
     6061 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc
     6121 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     6181 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     6241 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     6301 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     6361 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     6421 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     6481 tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg
     6541 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     6601 tggacgagct gtacaagtaa gcggccgcgg tccctgatgc cgtggaaagc agatactcta
     6661 gaacggaagg ctcccttgat ggaacttagg aattcgttag gccattaagg cctgtcgaca
     6721 agcggccgcc tcggccaaac atcgataaaa taaaagattt tatttagtct ccagaaaaag
     6781 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     6841 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     6901 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     6961 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     7021 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     7081 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     7141 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     7201 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     7261 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     7321 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
     7381 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
     7441 taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7501 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7561 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     7621 ggagtgtact gataacttcg tataatgtat gctatacgaa gttattaggt ctgaagagga
     7681 gtttacgtcc agccaagcta gggccgcgat ccggaaccct taatataact tcgtataatg
     7741 tatgctatac gaagttatca gtactggctt aactatgcgg catcagagca gattgtactg
     7801 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7861 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7921 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8461 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9121 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9421 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9781 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9841 aataggcgta tcacgaggcc ctttcgtctt caagaattgc tagcaattgc tagcaattgc
     9901 tagcaattca taccagatca ccgaaaactg tcctccaaat gtgtccccct cacactccca
     9961 aattcgcggg cttctgcctc ttagaccact ctaccctatt ccccacactc accggagcca
    10021 aagccgcggc ccttccgttt ctttgct
//
