LOCUS       sequence-64762-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Molyneaux BJ, Arlotta P, Fame RM, MacDonald JL, MacQuarrie KL, 
            Macklis JD
  TITLE     Novel subtype-specific genes identify distinct subpopulations of 
            callosal projection neurons.
  JOURNAL   J Neurosci. 2009 Sep 30;29(39):12343-54. doi: 
            10.1523/JNEUROSCI.6108-08.2009.
  PUBMED    19793993
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.6108-08"; journalName: "J Neurosci"; date: 
            "2009-09-30- 30"; volume: "29"; issue: "39"; pages: "12343-54"
COMMENT     Sequence Label: pCRII-Topo Chn2 in situ probe
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(579..598)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(580..598)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(605..622)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(605..621)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(614..636)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(849..868)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc tttttccttc cattacactg
      121 tcataaatta caaagtattg ctcacttcac aaaataaaac catttccaga ttattattga
      181 cagtatcaag aagaacagaa gccacaacaa acaaatctgt acagtcatga gggacagtga
      241 cagcagagag caagccaaac tgagccccag atgccacgat tctgcctgca gggcctgggg
      301 aggctaatag agcacttctg cagtctctat cactgtaaca gggcagtttt cttctgggca
      361 ctctaagcaa gacttgtcag tgtgtgggtc atctcagaca atcaagggaa agtcactggc
      421 tttaaaaatc taaatgtaca gtttttgctg ttttaaatat ttttgcctat gttacaaaat
      481 atagagatct tggtgtggaa atctcatgca agggcgaatt ctgcagatat ccatcacact
      541 ggcggccgct cgagcatgca tctagagggc ccaattcgcc ctatagtgag tcgtattaca
      601 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      661 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      721 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
      781 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      841 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      901 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      961 ccccaaaaaa cttgattagg g
//
