LOCUS       sequence-64764-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Molyneaux BJ, Arlotta P, Fame RM, MacDonald JL, MacQuarrie KL, 
            Macklis JD
  TITLE     Novel subtype-specific genes identify distinct subpopulations of 
            callosal projection neurons.
  JOURNAL   J Neurosci. 2009 Sep 30;29(39):12343-54. doi: 
            10.1523/JNEUROSCI.6108-08.2009.
  PUBMED    19793993
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.6108-08"; journalName: "J Neurosci"; date: 
            "2009-09-30- 30"; volume: "29"; issue: "39"; pages: "12343-54"
COMMENT     Sequence Label: pCRII-Topo Epha3 in situ probe
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(95..106)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(767..786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(768..786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(793..810)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(793..809)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(802..824)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttcaatagca tttggcactt
      121 ggctgtaaca aaacccaaat taaatcatca aggcataaat ataaaacaat ttaaaaatcc
      181 aaaagaaaat aaatacgcac ttgcccccaa attaagacgt gtttttacat ggtctactta
      241 aggaaggtaa caattaaaaa tgtatagtat ttaaaacaac tacaaacttt atctctatat
      301 gcttcaaaca gataaagaga atactaacgt gataaggtag taagtatggc cagatagttt
      361 gaggtataaa tgcctccatt ttgcttgttt ttaaaagaaa caaaatacct gttacagatt
      421 tttttctgtg tataaaaata cgacaagtct tttcctctgt ggccattcac aacaaagcct
      481 tcaggtctgt tttgctagtt tacttggttt atttccatga tagttaatga tcaatggctc
      541 tacactagtt cttccacttc atactgaaga attttaacta accccatcaa ttaacattta
      601 aaaagcaatt acaaagcaaa atgcacccac ccacccatct gtaaaatgat aggggaaaat
      661 aaagagattt ttcaattcca ttttaaggca tttggacaag ggcgaattct gcagatatcc
      721 atcacactgg cggccgctcg agcatgcatc tagagggccc aattcgccct atagtgagtc
      781 gtattacaat tcactggccg tcgttttaca acgtcgtgac tggg
//
