LOCUS       sequence-64772-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     Macklis lab in situ probes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCRII-Topo Cdh10 in situ probe
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(606..625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(607..625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(632..649)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(632..648)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(641..663)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttcgcctgtg ttatgttcaa
      121 cgagataaag ttaactttgc ggacactgtc tccacttcac aatatttgat attcaggaga
      181 aatttttttc ctgccactca gcacacgttt cccacttatt tcttaatttg ttcattaatg
      241 atattaattc tttcctgtag ggtgtctcat ggaatatata cgacatttta tttaatcact
      301 tccaagagcc aaagctatgg aaattcaatg ttgtccatct tagtaaataa aagagacctg
      361 agcaggatag ttctccctta agcaacctca cgaacaagcc gcttctgtta gatacacgtc
      421 ttgctcttgc aaatgaagct ttgataagac gaagaaaaca ctgaagatgt atcctgttct
      481 gtacattaag tttaaagaaa aaaatgtaca tgtggtgtta gttggtgtga tatgcaaagg
      541 gcgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca
      601 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
      661 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      721 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      781 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      841 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttc
//
