LOCUS       sequence-64774-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Balskus EP, Walsh CT
  TITLE     The genetic and molecular basis for sunscreen biosynthesis in 
            cyanobacteria.
  JOURNAL   Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637.
            Epub 2010 Sep 2.
  PUBMED    20813918
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1193637"; journalName: "Science"; date: 
            "2010-09-24- 24"; volume: "329"; issue: "5999"; pages: "1653-6"
COMMENT     Sequence Label: pET29b-NpR5599
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             877..899
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagta ccaagcgtct aatcagggtt
       61 ataccatctc gtatgggtaa cagaacttgc tctacacgag gatctgcggc gacaatgcgg
      121 ttgaattgag cgatcgcttc accattggct gtacgctgtt ctgatggcag gtaaacttgt
      181 ccttgcaaca aagtattatc tacacagatt aatccgtcgg gagctagtaa atggctatcc
      241 aagataattt ggaagtattc tatatactcc tttttatccg catcaatgaa gatcagatca
      301 aaggattctt ttttagccac cagcttgtgc agcgtctcta gtgcaggtgc cacttctaca
      361 acaatcttgc ggccgtgggg agactcttga aagcaagttt gagcaaactc ggccacatag
      421 gagtctactt cacaagcaat cagtcgccca tcatctggta acgcctctgc cattgccaaa
      481 gctgaatatc ctgtgaacat tcccacttct aggatgcttt tagccttagt gatatgcaca
      541 aacatcttta gtgtttgtcc ttcaagatgt cctgagagca tttcttgctc taattgacgc
      601 actgtttcac catcactgaa gcgtttactc cagtcttcaa tgcttgtctt ctgcgctagt
      661 gctgtcaatg cggtcgattc aggggtagtg caatcatcca gatagggatc taaacctgct
      721 gctaattgca acccttggcg cagagaagtc agtatctctg aaggaatatt actttcctct
      781 gccattctga gggttttttg tagctgtgct accagaatgc tatgtggcgt tattggtctt
      841 gcggtatctc gtcctaaaat actggtcata tgtatatctc cttcttaaag ttaaacaaaa
      901 ttatttctag agggg
//
