LOCUS       sequence-64777-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Balskus EP, Walsh CT
  TITLE     The genetic and molecular basis for sunscreen biosynthesis in 
            cyanobacteria.
  JOURNAL   Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637.
            Epub 2010 Sep 2.
  PUBMED    20813918
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1193637"; journalName: "Science"; date: 
            "2010-09-24- 24"; volume: "329"; issue: "5999"; pages: "1653-6"
COMMENT     Sequence Label: pET28b-Ava3855
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatgcagact atagatttta atattcgtaa
      121 gttacttgta gagtggaacg cgacccacag agattatgat ctttcccaga gtttacatga
      181 actaattgta gctcaagtag aacgaacacc tgaggcgatc gctgtcacct ttgacaagca
      241 acaactaact tatcaagaac taaatcataa agcaaaccag ctaggacatt atttacaaac
      301 attaggagtc cagccagaaa ccctggtagg cgtttgttta gaacgttcct tagaaatggt
      361 tatctgtctt ttaggaatcc tcaaagctgg gggtgcttat gttcctattg accctgaata
      421 tcctcaagaa cgcatagctt atatgctaga agattctcag gtgaaggtac tactaactca
      481 agaaaaatta ctcaatcaaa ttccccacca tcaagcacaa actatctgtg tagataggga
      541 atgggagaaa atttccacac aagctaatac caatcccaaa agtaatataa aaacggataa
      601 tcttgcttat gtaatttaca cctctggttc cactggtaaa ccaaaaggtg caatgaacac
      661 ccacaaaggt atctgtaatc gcttattgtg gatgcaggaa gcttatcaaa tcgattccac
      721 agatagcatt ttacaaaaaa ccccctttag ttttgatgtt tccgtttggg agttcttttg
      781 gactttatta actggcgcac gtttggtaat agccaaacca ggcggacata aagatagtgc
      841 ttacctcatc gatttaatta ctcaagaaca aatcactacg ttgcattttg tcccctcaat
      901 gctgcaagtg tttttacaaa atcgccatgt a
//
