LOCUS       sequence-64788-e        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Shcherbakova DM, Verkhusha VV
  TITLE     Near-infrared fluorescent proteins for multicolor in vivo imaging.
  JOURNAL   Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.
  PUBMED    23770755
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521."
COMMENT     Sequence Label: pMito-iRFP720
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..112
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcatg tccgtcctga cgccgctgct
       61 gctgcggggc ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca agatccattc
      121 gttgggggat ccaccggtcg ccaccatggc ggaaggatcc gtcgccaggc agcctgacct
      181 cttgacctgc gacgatgagc cgatccatat ccccggtgcc atccaaccgc atggactgct
      241 gctcgccctc gccgccgaca tgacgatcgt tgccggcagc gacaaccttc ccgaactcac
      301 cggactggcg atcggcgccc tgatcggccg ctctgcggcc gatgtcttcg actcggagac
      361 gcacaaccgt ctgacgatcg ccttggccga gcccggggcg gccgtcggag caccgatcac
      421 tgtcggcttc acgatgcgaa aggacgcagg cttcatcggc tcctggcatc gccatgatca
      481 gctcatcttc ctcgagctcg agcctcccca gcgggacgtc gccgagccgc aggcgttctt
      541 ccgccgcacc aacagcgcca tccgccgcct gcaggccgcc gaaaccttgg aaagcgcctg
      601 cgccgccgcg gcgcaagagg tgcggaagat taccggcttc gatcgggtga tgatctatcg
      661 cttcgcctcc gacttcagcg ggtccgtgat cgcagaggat cggtgcgccg aggtcgagtc
      721 aaaactaggc ctgcactatc ctgcctcatt catcccggcg caggcccgtc ggctctatac
      781 catcaacccg gtacggatca ttcccgatat caattatcgg ccggtgccgg tcaccccaga
      841 cctcaatccg gtcaccgggc ggccgattga tcttagcttc gccatcctgc gcagcgtctc
      901 gcccaaccat ctggagttca tgcgcaacat aggcatgcac ggcacgatgt cgatctcgat
      961 tttgcgcggc gagcgactgt ggggattgat cgtttgccat caccgaacgc cgtactacgt
     1021 cgatctcgat gg
//
